Key words: CD34+HSC humanized immune system reconstruction mice tumor immunotherapy preclinical efficacy test

　　Using CD34+ human hematopoietic stem cells isolated from human umbilical cord blood, the human immune system can be reconstructed in severely combined immunodeficiency mice. In this model, a complete human immune system was reconstructed, including human myeloid lymphocytes such as macrophages and DC cells, as well as lymphoid tumor cells such as T cells, B cells and NK cells.

　　Figure: Pedigree detection of human lymphocyte reconstruction in CD34+HSC humanized immune system reconstruction mice:

　　Such a model can be used to:

　　• NK related drug efficacy test.

　　• Bispecific antibody efficacy test.

　　• Testing of probiotics, Chinese medicine and other drugs that boost the immune system in ways that are not fully understood.

　　• Immunotherapy toxicology studies.

　　Although CD34+HSC has a more complete immune system reconstruction than PBMC models, such models are still limited.

　　• The volume of single donor cells is small, and the scale of the experiment is severely limited

　　• CD34+HSC mice constructed from different donors have different responses to immunotherapeutic drugs.

　　• Long build time and high cost

　　Compared with other NSG mice, NIG mice independently developed by Chuangmu Have higher radiation resistance. After higher dose of radiation, immunodeficient mice have better myeloid ablating effect and better homing and reconstruction effect of exogenous human immune cells. Therefore, fewer hematopoietic stem cells can be used to achieve stable immune system reconstruction, increasing the stability of reconstruction. Thus providing more single donor reconstruction animals.

　　CD34+HSC humanized immune system reconstruction animal models can effectively improve the performance of such models and greatly reduce the cost of such experiments. Related models have the following characteristics:

　　• A larger supply of single donor reconstructed mice.

　　• Reserve in advance and supply from spot.

　　• Reestablish stability and strictly check before use.

　　• Effective for the efficacy detection of NK related drugs and drugs that promote T lymphocyte efficacy through multiple pathways.

　　Based on the mature in vivo efficacy test platform and in vitro TIL flow analysis and cytokine detection test platform, The company can provide stable and efficient immunotherapy efficacy evaluation service using relevant models.

　　In addition to the use of self-produced CD34+HSC humanized immune system reconstruction mice to provide a variety of immunotherapy efficacy test services, model biology can also sell related laboratory animals, to provide more flexible support for your new drug research and development.

　　Contact us, our biological science support department will give you a detailed introduction of our experimental platform, we will assist you to choose the appropriate model for new drug development, and give you suggestions on experimental design based on our experience in tumor models. Follow us for professional tumor model support.