New Drug Development Services
Gouty arthritis (GA) model
Gout is a disease caused by the deposition of monosodium urate (MSU) crystals in joints, body fluids, and tissues due to high levels of uric acid in the blood. When deposited in the periarticular soft tissues and within the joints, it causes painful inflammation, known as gouty arthritis (GA), the most common manifestation of gout. This is characterized by red, swollen joints, acute and recurrent attacks, and can even lead to chronic arthritis and joint damage.
Case Presentation:
1. Modeling Method:
MSU deposition is a key factor in gouty arthritis. When MSU crystals deposit in the joints, they are recognized as "foreign bodies" by leukocytes. Leukocytes attempt to phagocytose these crystals, but this process triggers a series of inflammatory responses, releasing various inflammatory mediators such as cytokines and complement systems, leading to acute gout symptoms such as joint pain, swelling, redness, and fever. Therefore, gout animal models are mainly prepared by a single injection of MSU crystals into the joint cavity, which closely mimics clinical symptoms. This model can be used to study the in vivo pharmacodynamics of anti-gouty arthritis drugs.
2. Animal Species:
Adult female SD rats
3. Detection Indicators
(1) General observation;
(2) Joint swelling detection;
(3) Joint inflammation and impairment scoring (can be customized according to client needs);
(4) Analysis of TNF-α, IL-1β, IL-8, etc., in joint fluid (can be customized according to client needs);
(5) Joint tissue immunohistochemistry and pathological examination (can be customized according to client needs).
4. Results
(1) Body weight
(2) General observation
(3) Joint swelling (joint circumference):
Compared with before induction, both joints showed swelling, but the MSU-injected side showed more significant swelling. The swelling gradually returned to the pre-induction state after 24 hours.